Uranyl salts as photochemical agents for cleavage of DNA and probing of protein‐DNA contacts
- 1 August 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 235 (1-2) , 122-124
- https://doi.org/10.1016/0014-5793(88)81245-6
Abstract
Single-strand DNA nicks are induced by uranyl nitrate or uranyl acetate in combination with long-wavelength (λ ∼ 420 nm) ultraviolet irradiation. The nicks occur randomly with respect to the DNA sequence. Using the λ-repressor/OR1 operator DNA system it is shown that uranyl salts can be used to photofootprint protein contacts with the DNA backboneKeywords
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