IMMUNOPRECIPITATION OF BIOSYNTHETICALLY‐LABELLED PRODUCTS IN THE IDENTIFICATION OF ANTIGENS OF MURINE RED CELLS INFECTED WITH THE PROTOZOAN PARASITE, PLASMODIUM BERGHEI

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Abstract
In this methodological paper an immunoprecipitation technique has been optimised for the identification of antigens of Plasmodium berghei-infected blood which react with antibody specificities in a host-protective antiserum. Extracted 3H-leucine biosynthetically-labelled products of infected blood were sequentially reacted and precipitated with sera from mice which had been exposed to P. berghei but which were either non-protected or protected against lethal infection, protection having been shown to be transferable to naive recipients with the appropriate scrum. As analysed by polyacrylamide gel electrophoresis under reducing conditions, a small number of molecular species was detected in immunoprecipitates using host-protective sera which were apparently not quantitatively precipitated out of the complex mixture of labelled products of infected blood using sera from non-protected mice.