Characteristics of “Hybrid”-Type Clonal Cell Lines Obtained From Mixed Cultures In Vitro2

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Abstract
In confirmation of previous data, association in vitro of proliferating cultures of high-cancer N1- and low-cancer N2-type cells, derived from NCTC 2472 and NCTC 2555 clonal mouse cell lines, respectively, resulted in the appearance and accumulation of a new type of high polyploid “M” cells. Cells of this type were not observed in Nl or N2 cultures cultivated separately. Clones of M cells were obtained either directly from combined N1 + N2 cultures in vitro or from tumors produced by inoculation of C3H mice with mixed-cell populations. Karyotypes and biological behavior were similar for the 15 clonal M cell lines isolated from both origins. Some morphological and biological traits were common for the M and low-cancer N2 cells, but their malignancy approximated that of N1 cells. The “hybrid” characteristics of M cells remained stable during observation periods of nearly 1 year.