Stimulating Effect of Nucleoprotein Fraction of Chick Embryo Extract on Homologous Heart Fibroblasts.

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Abstract
A quantity isolation method is descr. for a chick embryo nucleoprotein fraction based on von Euler and Heller''s procedure for liver nucleoproteins. When this fraction was tested in an assay medium of undialyzed chicken plasma and dilute horse serum, large outgrowths of chick heart fibroblasts were obtained with well-defined alterations in cell morphology and mode of outgrowth. The nucleoprotein fraction descr. contains protein, 10.3% PNA, 0.3% DNA and has a characteristic nucleoprotein absorption spectrum. High polymer pentose nucleic acid isolated according to Nishioka and Ibuka produced no changes in size or appearance of assay cultures.