Establishment and characterization of a human T‐cell lymphoblastic lymphoma ceil line (HT‐1) carrying an inversion of chromosome 14

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Abstract
A new human lymphoblastic lymphoma cell line was established (designated HT‐1) from the pleural fluid lymphoma cells of a patient with lymphoblastic lymphoma of T‐cell type. The HT‐1 cells expressed CD1, CD2, CD3, CD4, CD5, CD7, CD8, CD57, and terminal deoxynucleotidyl transferase (TdT) but lacked B‐cell‐associated antigens and myeloid‐associated antigens. In addition, HT‐1 cells had rearranged T‐cell receptor (TCR) β‐chain gene and γ‐chain gene but retained germlines of immunoglobulin (Ig) heavy chain gene. These findings indicate that HT‐1 cell line represents a common thymocyte in the T‐cell lineage. Cytogenetic studies revealed that HT‐1 cells carry an inversion (inv) of the long arm of chromosome 14. This cell line is the second T‐cell line carrying inv(14) chromosome and may be useful for the molecular investigation of the cytogenetic break points of inv(14).