Studies on neplanocin a, new antitumor antibiotic. I. Producing organism, isolation and characterization.

Abstract
Neplanocin A, C11H13N5O3, is a novel carbocyclic analog of adenosine with cyclopentene. It was isolated from the culture filtrate of Ampullariella regularis A11079 by ion-exchange, carbon, silica gel adsorption or partition chromatography. Neplanocin A forms crystals and is stable at acidic or alkaline pH. Neplanocin A has cytotoxicity against [mouse lymphoma] L5178Y cells in culture and showed a remarkable effect on the life prolongation of mice infected with [mouse] L1210 leukemia cells.