Regulated expression of cloned human fetal Aγ‐globin genes introduced into murine erythroleukemia cells
- 1 September 1986
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 159 (3) , 475-478
- https://doi.org/10.1111/j.1432-1033.1986.tb09910.x
Abstract
We have analysed the expression of cloned human fetal .gamma.-globin genes introduced into murine erythroleukemia cells by a protoplast fusion procedure. Both the wild-type A.gamma.-globin gene and a mutant derivative related to a phenotype of hereditary persistence of fetal hemoglobin were studied. In both cases the level of .gamma.-globin mRNA increased by a factor of approximately sevenfold when erythroid cell differentiation was induced by treatment with hexamethylenebisacetamide. Thus, the regulation of the expression of the cloned fetal A.gamma.-globin gene in murine erythroleukemia cells resembled that of cloned adult .beta.-globin genes.This publication has 23 references indexed in Scilit:
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