Improved peptide sequencing using isotope information inherent in tandem mass spectra
- 27 June 2003
- journal article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 17 (15) , 1793-1801
- https://doi.org/10.1002/rcm.1119
Abstract
We demonstrate here the use of natural isotopic ‘labels’ in peptides to aid in the identification of peptides with a de novo algorithm. Using data from ion trap tandem mass spectrometric (MS/MS) analysis of 102 tryptic peptides, we have analyzed multiple series of peaks within LCQ MS/MS spectra that ‘spell’ peptide sequences. Isotopic peaks from naturally abundant isotopes are particularly prominent even after peak centroiding on y‐ and b‐series ions and lead to increased confidence in the identification of the precursor peptides. Sequence analysis of the MS/MS data is accomplished by finding sequences and subsequences in a hierarchical manner within the spectra. Copyright © 2003 John Wiley & Sons, Ltd.Keywords
This publication has 29 references indexed in Scilit:
- De novo peptide sequencing and quantitative profiling of complex protein mixtures using mass-coded abundance taggingNature Biotechnology, 2002
- Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometryNature, 2002
- Functional organization of the yeast proteome by systematic analysis of protein complexesNature, 2002
- Automated interpretation of low-energy collision-induced dissociation spectra by SeqMS, a software aid forde novo sequencing by tandem mass spectrometryElectrophoresis, 2000
- High Throughput Proteome-Wide Precision Measurements of Protein Expression Using Mass SpectrometryJournal of the American Chemical Society, 1999
- Protease‐catalyzed incorporation of 18O into peptide fragments and its application for protein sequencing by electrospray and matrix‐assisted laser desorption/ionization mass spectrometryElectrophoresis, 1996
- An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein databaseJournal of the American Society for Mass Spectrometry, 1994
- Identification of nearest-neighbor peptides in protease digests by mass spectrometry for construction of sequence-ordered tryptic mapsJournal of Mass Spectrometry, 1991
- Collisionally activated decomposition of modified peptides using a tandem hybrid instrumentJournal of Mass Spectrometry, 1988
- Preparation of stable isotope-incorporated peptide internal standards for field desorption mass spectrometry quantification of peptides in biologic tissueJournal of Mass Spectrometry, 1983