Antigenic sites of the nicotinic acetylcholine receptor cannot be predicted from the hydrophilicity profile
- 12 March 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 168 (1) , 143-148
- https://doi.org/10.1016/0014-5793(84)80224-0
Abstract
The amino acid sequences of the polypeptide chains of the acetylcholine receptor have recently been published. From the hydrophilicity profiles, it has been proposed that residues 161–166 of the α-chain might be an important antigenic site. We have synthesised a peptide containing this sequence and raised antisera to it. Here we report that this peptide does not represent an important antigenic site on the molecule, and that this region is probably inaccessible to antibodies. Based on the known DNA sequences and hydrophilicity profiles of the receptor chains, we suggest that many regions of high hydrophilicity may represent inter-domain regions of proteins.Keywords
This publication has 24 references indexed in Scilit:
- Cloning and sequence analysis of calf cDNA and human genomic DNA encoding α-subunit precursor of muscle acetylcholine receptorNature, 1983
- Structural homology of Torpedo californica acetylcholine receptor subunitsNature, 1983
- Primary structures of β- and δ-subunit precursors of Torpedo californica acetylcholine receptor deduced from cDNA sequencesNature, 1983
- Primary structure of α-subunit precursor of Torpedo californica acetylcholine receptor deduced from cDNA sequenceNature, 1982
- α‐Bungarotoxin binding to the nicotinic acetylcholine receptor is inhibited by two distinct subpopulations of anti‐receptor antibodiesEuropean Journal of Immunology, 1982
- Prediction of protein antigenic determinants from amino acid sequences.Proceedings of the National Academy of Sciences, 1981
- The Preparation of Merrifield‐Resins Through Total Esterification With Cesium SaltsHelvetica Chimica Acta, 1973
- Rapid solid-phase synthesis of bradykininBiochemical and Biophysical Research Communications, 1972
- The monitoring of reactions in solid-phase peptide synthesis with picric acidAnalytica Chimica Acta, 1972
- Solid Phase Peptide Synthesis. I. The Synthesis of a TetrapeptideJournal of the American Chemical Society, 1963