Detection of Insulin-Binding Antibodies and Separation of Free and Antibody-Bound Insulin by Rapid Chemical Procedure
- 1 March 1960
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 103 (3) , 542-544
- https://doi.org/10.3181/00379727-103-25589
Abstract
A rapid chemical method for detection of insulin-binding antibodies and separation of free and antibody-bound insulin in human antiserums is described. With NaCl-saturated 76% ethanol solution in 0.01 M KOH at −12°C to −10°C, 87% − 100% of antibody-bound insulin was precipitated from 15 of 18 insulin antiserums whereas 85% − 94% of unbound insulin in control serums remained in solution.Keywords
This publication has 3 references indexed in Scilit:
- QUANTITATIVE ASPECTS OF THE REACTION BETWEEN INSULIN AND INSULIN-BINDING ANTIBODYJournal of Clinical Investigation, 1959
- INSULIN-I131 METABOLISM IN HUMAN SUBJECTS: DEMONSTRATION OF INSULIN BINDING GLOBULIN IN THE CIRCULATION OF INSULIN TREATED SUBJECTS 1Journal of Clinical Investigation, 1956
- DISTRIBUTION AND METABOLISM OF I131 LABELED HUMAN SERUM ALBUMIN IN CONGESTIVE HEART FAILURE WITH AND WITHOUT PROTEINURIAJournal of Clinical Investigation, 1955