In vitro changes in blood P50 and erythrocyte 2,3-diphosphoglycerate concentration.
Open Access
- 1 July 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 22 (7) , 1073-1077
- https://doi.org/10.1093/clinchem/22.7.1073
Abstract
We studied in vitro changes in P50 and erythrocyte 2,3-diphosphoglycerate concentration occurring in blood 2 to 8 h after venipuncture. When blood was incubated at 37 degrees C, significant decreases in P50 were observed at 2, 4, and 8 hr. Such a change was significantly less when blood was kept at 4 degrees C. The rate of decrease in P50 was not changed when pH was altered by adding either lactic acid or sodium bicarbonate to the blood before incubation at 37 degrees C for 2 h. The erythrocyte 2,3-diphosphoglycerate concentration of blood incubated at 37 degrees C did not change by 2 h, but had significantly decreased by 4 h. To avoid in vitro changes, we recommend that P50 be determined as soon as possible for blood sampling.This publication has 5 references indexed in Scilit:
- Mixing technique for the oxygen-hemoglobin equilibrium and Bohr effectJournal of Applied Physiology, 1966
- Blood gas calculator.Journal of Applied Physiology, 1966
- Oxygen uptake and change in carbon dioxide tension in human blood stored at 37 CJournal of Applied Physiology, 1965
- Oxyhemoglobin dissociation curve in liver diseaseJournal of Applied Physiology, 1965
- Control of Concentration Gradients of Pyruvate and Lactate Across Cell Membranes in BloodJournal of Applied Physiology, 1956