The Photochemical Inactivation of Peptidyl Transferase Activity
- 1 October 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 58 (2) , 397-402
- https://doi.org/10.1111/j.1432-1033.1975.tb02386.x
Abstract
The photochemical oxidation of the 50‐S ribosomal subunit results in a rapid irreversible loss of peptidyl transferase activity. The first‐order rate of inactivation occurring during the first forty minutes suggests that a single reactive group is being inactivated. The pH profile of inactivation exhibits a maximum at pH 7.5.Erythromycin at a low concentration (0.04 μmol) affords significant protection. Puromycin also exerts a protective effect but at higher concentrations. Chloramphenicol, sparsomycin and lincomycin did not exert a protective effect.The loss in catalytic activity was not accompanied by a loss in substrate binding affinity of the donor and acceptor substrates.Keywords
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