Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult beta-globin gene.
Open Access
- 1 January 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (1) , 95-99
- https://doi.org/10.1073/pnas.81.1.95
Abstract
Chromatin in the 5'' flanking region of the transcriptionally active chicken adult .beta. (.beta.A) globin gene is hypersensitive to nuclease digestion. When plasmids carrying this gene are combined with histones in the presence of extracts from 9-day-old chicken erythrocyte nuclei, the resultant complex displays hypersensitive behavior in the same region. No such behavior is observed with extracts from cells in which the .beta.A-globin gene is not expressed. Partially purified preparations of the factor conferring hypersensitivity bind preferentially to DNA fragments containing the hypersensitive region.This publication has 21 references indexed in Scilit:
- A dominant role for DNA secondary structure in forming hypersensitive structures in chromatinCell, 1983
- Protein component from drosophila larval nuclei showing sequence specificity for a short region near a major heat-shock protein gene☆Cell, 1981
- Ultrasensitive Stain for Proteins in Polyacrylamide Gels Shows Regional Variation in Cerebrospinal Fluid ProteinsScience, 1981
- Tissue-specific exposure of chromatin structure at the 5' terminus of the rat preproinsulin II gene.Proceedings of the National Academy of Sciences, 1981
- Isolation and characterization of recombinant clones containing the chicken adult beta-globin gene.Journal of Biological Chemistry, 1979
- The chromatin structure of specific genes: I. Evidence for higher order domains of defined DNA sequenceCell, 1979
- A protein that preferentially binds Drosophila satellite DNA.Proceedings of the National Academy of Sciences, 1979
- Assembly of SV40 chromatin in a cell-free system from Xenopus eggsCell, 1977
- Chromosomal Subunits in Active Genes Have an Altered ConformationScience, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970