Binary ethylenimine as an inactivant for foot-and-mouth disease virus and its application for vaccine production
- 1 March 1975
- journal article
- research article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 47 (1) , 47-56
- https://doi.org/10.1007/bf01315592
Abstract
Foot-and-mouth disease virus was inactivated with binary ethylenimine formed apart from or directly in the virus suspension by the cyclization of 2-bromoethylamine hydrobromide or 2-chloroethylamine hydrochloride under alkaline conditions. The inactivation rates with binary ethylenimine prepared apart from the virus suspension in dilute sodium hydroxide with either 2-bromoethylamine hydrobromide or 2-chloroethylamine hydrochloride were higher than with pure ethylenimine. When binary ethylenimine was prepared directly in the virus suspension only 2-bromoethylamine hydrobromide gave acceptable inactivation rates. The reduced inactivation rates for binary ethylenimine directly prepared in the virus suspension are due to the different cyclization rates of 2-bromoethylamine hydrobromide and 2-chloroethylamine hydrochloride and to the interference of bicarbonate in the cyclization reaction. The complement fixing antigen of foot-and-mouth disease virus was not affected by binary ethylenimine inactivation. Vaccines prepared with foot-and-mouth disease virus inactivated by binary ethylenimine were comparable in their immunogenicity to vaccines prepared with ethylenimine or N-acetylethylenimine used as inactivants. Application of binary ethylenimine in the preparation of foot-and-mouth disease vaccines considerably reduces the potential danger associated with handling pure ethylenimine and other aziridines.Keywords
This publication has 22 references indexed in Scilit:
- Inactivation of foot-and-mouth disease virus with ethylenimine.1973
- Derivatives of aziridine as inactivants for foot-and-mouth disease virus vaccines.1973
- The Inactivation of Foot‐and‐Mouth Disease Virus by Ethylenimine and PropylenimineZentralblatt für Veterinärmedizin Reihe B, 1973
- [The inactivation of foot-and-mouth disease virus by ethylethyleneimine and the use of the inactivated virus in the preparation of vaccines].1970
- HANDLING AND STORAGE OF AZIRIDINESPublished by Elsevier ,1969
- The use of acetylethyleneimine in the production of inactivated foot-and-mouth disease vaccinesEpidemiology and Infection, 1963
- INACTIVATION OF HOG CHOLERA VIRUS : II. INACTIVATION WITH NITROGEN MUSTARD COMPOUNDSThe Japanese Journal of Veterinary Science, 1961
- INACTIVATION OF VIRUSES AND CELLS BY MUSTARD GASThe Journal of general physiology, 1948
- Inactivation of Influenza Virus with Sulfur and Nitrogen MustardsExperimental Biology and Medicine, 1947
- The Biological Actions and Therapeutic Applications of the B-Chloroethyl Amines and SulfidesScience, 1946