Cholesterol requirement of mycoplasmas as determined by microtiter test using polyene antibiotics

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Abstract
A microtiter metabolic inhibition test was used to determine the effect of filipin and lucensomycin on the growth of representative species of Mycoplasma and Acholeplasma. The cholesterol-requiring species tested were very susceptible to the 2 antibiotics; the cholesterol nonrequiring species were not. Utilization of this method for differentiation of Mycoplasma and Acholeplasma is suggested and its advantages are discussed.