Characterization of recombinant mustard trypsin inhibitor 2 (MTI2) expressed in Pichia pastoris
Open Access
- 22 February 2000
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 468 (2-3) , 137-141
- https://doi.org/10.1016/s0014-5793(00)01207-2
Abstract
The mustard trypsin inhibitor MTI2 was expressed as secretory protein in the yeast Pichia pastoris. In order to evaluate the influence of the C‐terminal amino acids of the precursor form on the inhib...Keywords
This publication has 17 references indexed in Scilit:
- The preparation and properties of two new chromogenic substrates of trypsinPublished by Elsevier ,2004
- Opposite Effects on Spodoptera littoralis Larvae of High Expression Level of a Trypsin Proteinase Inhibitor in Transgenic PlantsPlant Physiology, 1998
- The gene coding for the mustard trypsin inhibitor‐2 is discontinuous and wound‐inducibleFEBS Letters, 1995
- Purification, inhibitory properties, amino acid sequence and identification of the reactive site of a new serine proteinase inhibitor from oil‐rape (itBrassica napus) seedFEBS Letters, 1994
- Quantitative Determination of Serine Proteinase Inhibitor Activity Using a Radial Diffusion AssayAnalytical Biochemistry, 1993
- Purification, inhibitory properties and amino acid sequence of a new serine proteinase inhibitor from white mustard (Sinapis alba L.) seedFEBS Letters, 1992
- Protease Inhibitors in Plants: Genes for Improving Defenses Against Insects and PathogensAnnual Review of Phytopathology, 1990
- Isolation and characterization of a trypsin inhibitor from white mustard (Sinapis alba L.)Journal of Agricultural and Food Chemistry, 1985
- Isolation and characterization from potato tubers of two polypeptide inhibitors of serine proteinasesArchives of Biochemistry and Biophysics, 1982
- p-Nitrophenyl-p′-guanidinobenzoate HCl: A new active site titrant for trypsinBiochemical and Biophysical Research Communications, 1967