Characterization of Antibodies Against Human N-Terminal Parathyroid Hormone by Epitope Mapping
- 1 March 1992
- journal article
- research article
- Published by Taylor & Francis in Journal of Immunoassay
- Vol. 13 (1) , 1-13
- https://doi.org/10.1080/15321819208019821
Abstract
Two polyclonal antisera from goat and mouse and two monoclonal antibodies against human parathyroid hormone (1–34) were characterised by epitope mapping. Hexapeptides were synthesized on polystyrene pins, the sequences of which overlapped and represented the entire sequence of hPTH(1–34). Binding of antibodies to these hexapeptides was determined and antigenic determinants thus characterized. At least one predominant binding sequence was detected in the region of hPTH(7–14).Keywords
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