Purification of Human Interferon by Antibody Affinity Chromatography, using Highly Absorbed Anti‐interferon

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Abstract
Antibodies against partially purified human leucocyte interferon (PIF) were bound to Sepharose 4B and crude interferons applied on this affinity column were purified, up to 8 × 105 interferon units (IFU) per mg protein in one step. Antibodies against PIF were absorbed with immobilized crude human leucocyte interferon bound to Sepharose, whereby antibodies against impurities were predominantly removed. Extensively absorbed antisera were coupled to Sepharose and used for antibody affinity chromatography of crude interferon preparations. Leucocyte and fibroblast interferons were purified in one step with around 100% recovery, up to 1 × 108 IFU per mg protein, and Namalva interferon up to 2 × 107 IFU/mg. SDS electrophoresis of affinity‐purified leucocyte interferon revealed that the interferon activity appeared in two bands (19,000 and 23,000 D).