Creatine Transport in Cultured Cells of Rat and Mouse Brain
- 1 February 1989
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 52 (2) , 544-550
- https://doi.org/10.1111/j.1471-4159.1989.tb09154.x
Abstract
Astroglia‐rich cultures derived from brains of newborn rats or mice use a transport system for the uptake of creatine. The uptake system is saturable, Na+‐dependent, and highly specific for creatine and Na+. Kinetic studies on rat cells revealed a Km value for creatine of 45 μM, a Vmax of 17 nmol X h−1 X (mg of protein)−1, and a Km value of 55 mM for Na+. The carrier is competitively inhibited by guanidinopropionate (Ki= 15 μM). No such transport system was found in neuron‐rich primary cultures from embryonic rat brain. It is hypothesized that creatine transport is an astroglial rather than a neuronal function.Keywords
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