Coagulation Activities in Perfused Organs: Regulation by Addition of Animal Plasmas

Publisher Website
Google Scholar
Abstract
Summary. Production of factor‐VIII and ‐IX activities was studied in isolated rabbit livers and spleens perfused for I hr to release stored coagulation activity and then for another 4 hr. At 30 min of the second perfusion period, 5 ml citrated dog or rabbit plasma from normal animals or from those with congenital or artificially induced coagulation defects was added to the perfusates. Subsequent production of factor‐VIII and ‐IX activities was monitored at intervals for 3.5 hr. Results indicated that following the addition of animal plasmas to perfused organs, production of coagulation activity was inversely proportional to the level of these coagulation factors in the initial perfusate. In addition, development of clotting factor activities in the liver and spleen perfusates was inhibited by puromycin, cycloheximide, and actinomycin D. Several interpretations of the data have been considered, and the results are compatible with regulation by a negative feedback effect on organ protein synthesis and/or release of previously formed molecules.