CHEMICALLY DEFINED MEDIUM FOR SOME MICROAEROPHILIC VIBRIOS

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Abstract
The medium described supported growth of 22 of 26 catalase-positive H2S-negative vibrios (Vibrio fetus). The strains used were isolated from cattle, chickens, man, sheep and swine; 10 failed to grow in thiol medium plus 1% glycine (group I), and 16 were glycine-tolerant (group n). Growth on the defined medium was compared with growth on yeast extract agar, blood agar and thiol agar. Four of the group I strains failed to grow on the defined medium, and growth of seven of the group II strains equaled or exceeded growth obtained on the compared media. Use of synthetic media for determining growth requirements and for possible classification of vibrios is discussed.