On-Column Propylation Method for Measuring Plasma Valproate Concentrations by Gas Chromatography

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Abstract
A gas-chromatographic method is described for measuring plasma valproate concentrations. This method incorporates double-solvent extraction of the plasma sample and on-column propylation of valproic acid and 2-propylhexanoic acid, used as the internal standard. The identity of the propylester derivatives of these compounds was confirmed by gas chromatographymass spectrometry. The accuracy and simplicity of the method make it suitable for routine laboratory use. The assay is sufficiently rapid that a plasma sample can be analyzed in duplicate within 30 min.