Characterization of nuclear structures containing superhelical DNA
Open Access
- 1 November 1976
- journal article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 22 (2) , 303-324
- https://doi.org/10.1242/jcs.22.2.303
Abstract
Structures resembling nuclei but depleted of protein may be released by gently lysing cells in solutions containing non-ionic detergents and high concentrations of salt. These nucleoids sediment in gradients containing intercalating agents in a manner characteristic of DNA that is intact, supercoiled and circular. The concentration of salt present during isolation of human nucleoids affects their protein content. When made in I-95 M NaCl they lack histones and most of the proteins characteristic of chromatin; in 1-0 M NaCl they contain variable amounts of histones. The effects of various treatments on nucleoid integrity were investigated.Keywords
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