Vascular volume and blood level of antidiuretic hormone

Publisher Website
Google Scholar
Abstract
The role of vascular volume in regulation of the blood level of antidiuretic hormone (ADH) was investigated utilizing a chemical procedure for extraction, isolation, and concentration of ADH in blood. The antidiuretic material recovered from blood was identified as ADH on the basis of its biological activity and the results of a number of chemical tests. Reduction in extracellular fluid volume by peritoneal lavage with hypertonic solution produced a fourfold increase in the blood titer of ADH within 20 min. Expansion of vascular volume at that time by the intravenous infusion of isotonic dextran solution resulted in a 50% reduction in the blood level of ADH. This reduction was maintained for the 30-min period of observation, although the blood level of ADH rose progressively when the lavage procedure was uninterrupted by infusion of dextran solution. It is concluded that blood volume is an important factor in regulation of blood concentration of ADH.