On the Nature of Cytoplasmic Inclusions of Nocardia rubra

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Abstract
A cytological investigation of cell walls, lipid granules, metachromatic granules and chromatinic bodies of Nocardia rubra revealed that each of these structures existed as separable entities. Multicellularity was not observed as indicated by the lack of septa in non-fragmenting organisms, but lipid inclusions were frequently found in close association with cell walls. Metachromatic granules were separable from lipid inclusions and were found to be homologous with electron opaque granules. No evidence for the origin of metachromatic granules as cytoplasmic condensations upon cell septa was found. Acid hydrolysis or ribonuclease treatment revealed Giemsa-staining chromatinic structures which were removed by deoxyribonuclease digestion. Metachromatic granules were not removed by ribonuclease, doexyribonuclease hydrolysis or a combination of both; but these granules were removed by acid hydrolysis.

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