Thermolysin Is a Suitable Protease for Probing the Surface of Intact Pea Chloroplasts
- 1 July 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 75 (3) , 675-678
- https://doi.org/10.1104/pp.75.3.675
Abstract
Several proteases, i.e., pronase, a mixture of trypsin and chymotrypsin, and thermolysin were screened as potential surface probes of isolated intact pea chloroplasts. Of these, only thermolysin met the criteria of a suitable probe. Thermolysin destroyed outer envelope polypeptides, but did not affect inner envelope polypeptides, envelope permeability properties or such chloroplast activities as metabolite transport and O2 evolution.This publication has 9 references indexed in Scilit:
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