Studies on xylanase fromBasidiomycetes

Abstract
Formation of extracellular xylanase was studied in 10 strains of wood-destroying fungi belonging toBasidiomycetes during their submerged cultivation with willow sawdust. The highest enzyme activity was found in the fungusTrametes hirsuta (Wulf.) Pilát. The effect of sources of carbon and nitrogen, cultivation time and initial pH of the cultivation solution on the formation of xylanase by the fungusTrametes hirsuta was investigated. The highest production of the enzyme was reached during cultivation in the presence of willow sawdust, asparagine and at the initial pH of 5.0. The presence of xylanase, cellulase, mannanase and amylase as well as of beta-xylosidase, beta-glucosidase, beta-mannosidase and betagalactosidase was demonstrated in the enzyme preparation obtained after a 10-day submerged cultivation ofTrametes hirsuta under optimal conditions.

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