Iodine-125—Labeled Antibody to Viral Antigens: Binding to the Surface of Virus-Infected Cells
- 5 May 1972
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 176 (4034) , 516-518
- https://doi.org/10.1126/science.176.4034.516
Abstract
The specific binding of iodine-125-labeled antibody to viral antigens can be used to detect newly synthesized viral antigens and determine the time of appearance of these antigens on the surface of infected cells. Incubation of infected cells with unlabeled antibody to viral antigens specifically blocks the attachment of labeled antibody, and by this inhibition technique the titer of unlabeled antibody to viral antigens can be calculated. The attachment of the labeled antibody to virus-infected cells offers an objective and sensitive method of detecting viral antigens and measuring antibody to virus.Keywords
This publication has 10 references indexed in Scilit:
- A specific surface antigen induced by poxvirusVirology, 1969
- SMALLPOX VACCINATION OF ECZEMA PATIENTS WITH A STRAIN OF ATTENUATED LIVE VACCINIA (CVI-78)Pediatrics, 1968
- RELATION BETWEEN EPSTEIN-BARR VIRAL AND CELL MEMBRANE IMMUNOFLUORESCENCE OF BURKITT TUMOR CELLSThe Journal of Experimental Medicine, 1968
- Sequential Protein Synthesis Following Vaccinia Virus InfectionJournal of Virology, 1968
- Heterogeneity in the Properties of 7 S and 19 S Rabbit-Neutralizing Antibodies to Herpes Simplex VirusThe Journal of Immunology, 1968
- Soluble Antigens of Vaccinia-infected Mammalian Cells II. Time Course of Synthesis of Soluble Antigens and Virus Structural ProteinsJournal of Virology, 1967
- Application of the Paired Label Radioantibody Technique to Tissue Sections and Cell SmearsThe Journal of Immunology, 1967
- A Method of Trace Iodination of Proteins for Immunologic StudiesInternational Archives of Allergy and Immunology, 1966
- Studies on “early” enzymes in HeLa cells infected with vaccinia virusVirology, 1965
- Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific ActivityNature, 1962