THE REACTIVITY OF HUMAN PLATELETS IN THE 125I‐LABELLED STAPHYLOCOCCAL PROTEIN A TEST

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Abstract
Stored platelets were employed in the testing of various antiplatelet antibodies and platelet unrelated immune complexes. The antisera included sera containing ABO-, HLA- or platelet specific (Zwa) antibodies and serum from an adult patient witb idiopathic thrombocytopenic purpura (ITP). Use was made of 125I-labeled staphylococcal protein A (IPA) for the detection of platelet bound Ig[immunoglobulin]G. The reactivity of cells with the ITP serum and sera from healthy blood donors increased after storage for a few days, and declined after a highly reactive period extending over some weeks. In some cases predilution of the serum increased the probability of discovering weak antibodies. The normal components of the serum modified the platelet-antibody reaction, but all the various kinds of platelet reactive activities could be detected by this single test.