Some Factors that Influence Utilization of Antihemophilic Activity During Clotting.

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Abstract
Antihemophilic factor (AHF) does not disappear after recalcification of plasma which has been absorbed with BaSO4 as it does from recalcified normal plasma. AHF was thus stabilized in plasma by BaSO4 absorption and the effects of various plasmas, sera, or fractions on AHF utilization were studied. These experiments revealed that a source of thrombin is required for AHF utilization. Purified prothrombin, when activated by either cephalin or by 25% sodium citrate, caused loss of AHF in proportion to the prothrombin or thrombin concentration, within the 0.25-5.0 [mu]/ml range. Calcium ions accelerated the inactivation of AHF caused by purified thrombin.