Utilization of the Antihemophilic Factor During Clotting of Canine Blood and Plasma

Abstract
Using prothrombin conversion in canine hemophilic blood as an index of the antihemophilic activity of added normal plasma or serum, the rate of disappearance of AHF (antihemophilic factor) in clotting blood and plasma under varying circumstances was studied. Prothrombin and AHF disappeared at equal rates, each being proportional to the platelet level and available wettable surface. The utilization appeared to depend on the presence of ionic Ca and prothrombin, but to be independent of fibrinogen.