Ion channels from the Bacillus subtilis plasma membrane incorporated into planar lipid bilayers
- 26 October 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 311 (3) , 246-250
- https://doi.org/10.1016/0014-5793(92)81112-y
Abstract
Fusion of Bacillus subtilis plasma membrane vesicles with planar lipid bilayers induced the appearance of discrete current fluctuations characteristic of ion channels. These channels showed a wide range of conductances and kinetic behaviors. In 300 mM KCl, their conductances ranged from a few hundreds of pS to more than 1 nS, and most of them exhibited several sub‐states. The channels poorly discriminated between small univalent anions and cations. Some of them showed voltage dependence and most of them presented a complex gating kinetics. The results are consistent with the hypothesis of the presence in the B. subtilis plasma membrane of pores composed of subunits that function cooperatively.Keywords
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