Hydrolysis of Nerve Gas by Squid-Type Diisopropyl Phosphorofluoridate Hydrolyzing Enzyme on Agarose Resin
- 5 March 1982
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 215 (4537) , 1255-1257
- https://doi.org/10.1126/science.7058344
Abstract
An enzyme purified from squid nerve that hydrolyzes the cholinesterase inhibitor diisopropyl phosphorofluoridate (DFP) has now been coupled to agarose beads. A column of this agarose-DFPase hydrolyzes the nerve gas 1,2,2-trimethylpropyl methylphosphonofluoridate (Soman). Although the more inhibitory of the four diastereoisomers of Soman are hydrolyzed least rapidly, a column of sufficient length will accomplish 95 percent hydrolysis whether measured by fluoride release or loss of cholinesterase-inhibiting power. The results suggest a means for detoxifying unwanted chemical warfare agents.This publication has 14 references indexed in Scilit:
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