PROTEOLYTIC DEGRADATION OF STAPHYLOCOCCAL α-TOXIN
- 15 August 2009
- journal article
- research article
- Published by Wiley in Acta Pathologica Microbiologica Scandinavica Section B Microbiology
- Vol. 84B (5) , 309-314
- https://doi.org/10.1111/j.1699-0463.1976.tb01943.x
Abstract
Staphylococcal [Staphylococcus aureus] .alpha.-toxin of MW 39,000 was degraded at an alkaline pH by staphylococcal extracellular proteases resulting in the formation of 3 relatively stable intermediates with MW 27,500, 23,500 and 12,000. The intermediate with MW 27,500, which existed in 2 charged forms, was isolated by column chromatography and found to be non-hemolytic. It could be obtained by proteolysis of .alpha.-toxin (MW 39,000) with chymotrypsin in low concentrations. This intermediate was further degraded by trypsin to the protein with MW 23,500 and 12,000.Keywords
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