Plasma and Urine Responses Are Lower for Acylated vs Nonacylated Anthocyanins from Raw and Cooked Purple Carrots

Abstract
The bioavailability of acylated vs nonacylated anthocyanins and the effect of cooking and dose on the comparative bioavailability were investigated in a clinical feeding study using purple carrots as the anthocyanin source. Treatments were purple carrots as follows: 250 g raw (463 μmol of anthocyanins: 400 μmol acylated, 63 μmol nonacylated), 250 g cooked (357 μmol of anthocyanins: 308.5 μmol acylated, 48.5 μmol nonacylated), and 500 g cooked (714 μmol of anthocyanins: 617 μmol acylated, 97 μmol nonacylated). Four of the five carrot anthocyanins were found intact in plasma by 30 min after carrot consumption and peaked between 1.5 and 2.5 h. Acylation of anthocyanins resulted in an 11−14-fold decrease in anthocyanin recovery in urine and an 8−10-fold decrease in anthocyanin recovery in plasma. Cooking increased the recovery of nonacylated anthocyanins but not acylated anthocyanins. Large dose size significantly reduced recovery of both acylated and nonacylated anthocyanins, suggesting saturation of absorption mechanisms. Keywords: Anthocyanin; bioavailability; absorption; carrot (Daucus carota)

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