Slowing DNA Translocation in a Solid-State Nanopore
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- 9 August 2005
- journal article
- letter
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 5 (9) , 1734-1737
- https://doi.org/10.1021/nl051063o
Abstract
Reducing a DNA molecule's translocation speed in a solid-state nanopore is a key step toward rapid single molecule identification. Here we demonstrate that DNA translocation speeds can be reduced by an order of magnitude over previous results. By controlling the electrolyte temperature, salt concentration, viscosity, and the electrical bias voltage across the nanopore, we obtain a 3 base/μs translocation speed for 3 kbp double-stranded DNA in a 4−8 nm diameter silicon nitride pore. Our results also indicate that the ionic conductivity inside such a nanopore is smaller than it is in bulk.Keywords
This publication has 24 references indexed in Scilit:
- Sizing DNA Using a Nanometer-Diameter PoreBiophysical Journal, 2004
- DNA-Mediated Fluctuations in Ionic Current through Silicon Oxide Nanopore ChannelsNano Letters, 2004
- DNA molecules and configurations in a solid-state nanopore microscopeNature Materials, 2003
- Fabrication of solid-state nanopores with single-nanometre precisionNature Materials, 2003
- Ion-beam sculpting at nanometre length scalesNature, 2001
- Voltage-Driven DNA Translocations through a NanoporePhysical Review Letters, 2001
- Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channelNature Biotechnology, 2001
- Rapid nanopore discrimination between single polynucleotide moleculesProceedings of the National Academy of Sciences, 2000
- Microsecond Time-Scale Discrimination Among Polycytidylic Acid, Polyadenylic Acid, and Polyuridylic Acid as Homopolymers or as Segments Within Single RNA MoleculesPublished by Elsevier ,1999
- Characterization of individual polynucleotide molecules using a membrane channelProceedings of the National Academy of Sciences, 1996