Structural Studies of 2-Thiouridine in RNA
- 1 July 1997
- journal article
- research article
- Published by Taylor & Francis in Nucleosides and Nucleotides
- Vol. 16 (7-9) , 1469-1472
- https://doi.org/10.1080/07328319708006208
Abstract
A pentamer RNA sequence, Gs2UUUC, and a s2U containing 14-mer RNA tetraloop hairpin were synthesized and characterized by NMR and by UV melting studies. These oligonucleotides were used as models to understand the effect of 2-thiouridine substitution on RNA structure and the potential for stabilization of tRNA codon-anticodon interactions through sU-34 modification. The magnitude of the effect of sU in our model system is comparable to the 20° C stabilization reported for 2-thiolation in a codonanticodon model system composed of two tRNAs with complementary anticodon sequences.Keywords
This publication has 9 references indexed in Scilit:
- Modified Nucleosides and Codon Recognition+Published by American Society for Microbiology ,2014
- Synthesis of Oligoribonucleotides Containing 2-Thiouridine: Incorporation of 2-Thiouridine Phosphoramidite without Base ProtectionThe Journal of Organic Chemistry, 1995
- An Efficient Method for the Isolation and Purification of OligoribonucleotidesNucleosides and Nucleotides, 1995
- Thiolation of uridine carbon-2 restricts the motional dynamics of the transfer RNA wobble position nucleosideJournal of the American Chemical Society, 1992
- Effect of Uridine Dethiolation in the Anticodon Triplet of tRNA(Glu) on its Association with tRNA(Phe)Journal of Biomolecular Structure and Dynamics, 1988
- Chemistry and structure of modified uridines in the anticodon, wobble position of transfer RNA are determined by thiolationJournal of the American Chemical Society, 1987
- DNA and RNA: NMR studies of conformations and dynamics in solutionQuarterly Reviews of Biophysics, 1987
- Synthesis and hybridization studies on two complementary nona(2'-O-methyl)ribonucleotidesNucleic Acids Research, 1987
- Principles of Nucleic Acid StructurePublished by Springer Nature ,1984