Secretion of Chemotaxins by Guinea Pig Lung Macrophages I. The Spectrum of Inflammatory Cell Responses

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Abstract
Chemotactic activity for neutrophils, macrophages, and lymphocytes was detected in the medium of guinea pig lung macrophage cultures. For the phagocytic cells, activity was greatest for neutrophils and least for lung macrophages. Activity for peritoneal macrophages was intermediate. Although the response of lymphocytes could not be directly compared to that of phagocytes, migration of lymphocytes toward culture supernatant greatly exceeded migration toward fresh medium. A phagocytic stimulus enhanced the activity for neutrophils in medium from cultures incubated for 2 hr. By 24 hr of incubation, however, activity in medium of stimulated and unstimulated cultures was similar. Since the increase in activity occurring over an 18 hr culture incubation period was not associated with loss of cell viability, it appears that release of chemotactic activity is a specific physiologic event. The finding that cultured lung macrophages secrete potent chemoattractants for neutrophils, macrophages, and lymphocytes in vitro lends support to the notion that this cell initiates both acute and chronic inflammatory reactions in vivo.