Heterogeneity of locomotion in human T cell subsets.

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Abstract
Locomotor activity of T [thymus-derived] cells with receptors for Ig[immunoglobulin]M and IgG, T cells without receptors for IgM or IgG, and T and non-T cells from human peripheral blood and human tonsils towards the chemoattractant casein was examined in modified Boyden chambers. T cells with receptors for IgG from human tonsils and peripheral blood did not move in response to casein. T cells with receptors for IgM and those without receptors for IgM or IgG moved very well toward casein and the distances were comparable to those achieved by T cells before separation. This difference in the locomotor activity of T cell subsets might explain their differential distribution in various lymphoid compartments. Separated T cells, cultured in medium supplemented with fetal calf serum, moved into the filters in response to casein. Prior culture of T cells in medium alone or in medium supplemented with human AB serum resulted in a reduction in the distance traveled in respnse to casein; however, the effect of AB serum was variable. Non-T cells from peripheral blood and B [bone marrow-derived] cells from tonsils responded poorly to casein.