Abstract
A detailed account of diakinesis with figures is given for 11 mutants, hybrid derivatives, segregates and hybrids of Oenothera. Additional evidence to that given in 1929 is presented, based upon all available cytological studies so far made on Oenothera, to show that chromosomal configurations at diakinesis are only relatively constant features of the genus. Since in no instance, where the chromosomal configurations were found to be variable, were there observed any genotypical changes corresponding to these variable configurations, it is doubtful whether closed circles represent linkage groups. An indeterminate arrangement of the chromosomes in closed circles is indicated because of certain evidence presented. There is considerable evidence offered which throws doubt upon the assumption that closed circles result from heterozygosity. Furthermore the idea is supported that chromosomal configurations are inherited traits and as such are the result of gene action or interaction.

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