PSEUDOMONAS AERUGINOSA BACTERIOPHAGE SD1

Publisher Website
Google Scholar
Abstract
A DNA-containing bacteriophage, designated SD1, was isolated from sewage, using strain B71 of Pseudomonas aeruginosa as host. Lysates titering 1 to 2 × 1011plaque-forming units/ml are produced by infecting cultures growing in a defined medium. Highly purified phage preparations were obtained by a procedure involving concentration with ammonium sulfate at pH 8.2, differential centrifugation, and density gradient centrifugation in cesium chloride solution.Electron microscopy revealed a structure possessing a head of regular hexagonal outline, 50 mμ in diameter, and a tail, 6.2 × 188 mμ. The phage contained approximately 2.8 × 10−17 g nitrogen, 8 × 10−18 g of phosphorus, and 1.1 × 10−16 g DNA per plaque-forming unit. Base analysis of SD1 DNA disclosed the presence of equimolar amounts of adenine and thymine and of guanine and cytosine; the latter two comprise 53% of the bases. The thermal denaturation profile of the isolated DNA indicates that SD1 DNA is a highly ordered structure: the guanine and cytosine content as estimated from the temperature of half maximal ultraviolet absorption agrees with that found by chemical analysis of the DNA.

This publication has 27 references indexed in Scilit: