Scanning Electron Microscopic Studies of the Life Cycle of Ichthyophthirius multifiliis

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Abstract
All stages in the life cycle of the holotrichous cilitate Ichthyophthirius multifiliis were examined using the scanning electron microscope. Different procedures were used to prepare the tomite, trophozoite and cyst stages. Photomicrographs of freeze dried and critical point dried organisms fixed with glutaraldehyde are presented along with views of trophozoites and tomites deciliated by dibucaine hydrocholoride to reveal the pellicle and buccal cavity normally covered by cilia.