The Metabolism of 14C-Glycine and 14C-Bicarbonate by Washed Suspensions of the Rumen Ciliate Entodinium caudatum

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Abstract
Washed suspensions of Entodinium caudatum incubated anaerobically in the presence of penicillin and neomycin incorporated 14C-glycine in the cell ‘pool’ as N-acetylglycine and into the cell protein as glycine without conversion to any other amino acid. At low external concentrations the glycine was present in the protozoa themselves and was not free in the gastric sac or present solely in the intracellular or extracellular bacteria. The incorporation reached a constant value after incubation for 24 hr; after this time the uptake of glycine was balanced by an equal loss of N-acetylglycine from the organisms. These suspensions also incorporated 14C from NaH14CO3 into carbon atoms 3 and 4 of protozoal glucose which was probably present as starch in the intact protozoa.