The Preparation and Survival of Almost Bacteria-free Suspensions of Entodinium caudatum

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Abstract
SUMMARY: Suspensions of Entodinium caudatum containing less than one viable bacterium per ten protozoa were prepared by aerobic incubation of the protozoa with penicillin, streptomycin, dihydrostreptomycin and neomycin in the presence of autoclaved rumen fluid and rice starch grains. Unfortunately these almost axenic Entodinia could be maintained alive for only 3-4 days. Replacement of the rice starch grains in the medium used for bacterial removal by soluble starch produced almost bacteria-free protozoa which were devoid of food storage materials and died more rapidly than usual. The life of these protozoa was prolonged by adding any of the following: rice starch grains, soluble starch, maltose, glucose and some other sugars.
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