Primary structure of the nuclear PUT2 gene involved in the mitochondrial pathway for proline utilization in Saccharomyces cerevisiae.
Open Access
- 1 December 1984
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 4 (12) , 2837-2842
- https://doi.org/10.1128/mcb.4.12.2837
Abstract
The PUT2 gene, believed to encode delta 1-pyrroline-5-carboxylate dehydrogenase, has been completely sequenced. The DNA contains an open reading frame of 1,725 base pairs encoding a protein of 575 amino acids. Transcript mapping with both S1 nuclease and primer extension methods revealed numerous initiation sites of RNA synthesis 50 to 80 base pairs downstream from several TATA boxes. The deduced amino acid sequence of delta 1-pyrroline-5-carboxylate dehydrogenase contains a highly basic amino terminus that may serve as the signal sequence that targets this protein to the mitochondrion.This publication has 41 references indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- Yeast gene TRP5: structure, function, regulation.Journal of Biological Chemistry, 1982
- Organization and Expression of Eucaryotic Split Genes Coding for ProteinsAnnual Review of Biochemistry, 1981
- Identification of mitochondrial proteins and some of their precursors in two-dimensional electrophoretic maps of human cells.Proceedings of the National Academy of Sciences, 1981
- The sequence 5′-AAUAAA-3′ forms part of the recognition site for polyadenylation of late SV40 mRNAsCell, 1981
- A position effect in the control of transcription at yeast mating type lociNature, 1981
- Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomesNucleic Acids Research, 1981
- Intracellular protein topogenesisProceedings of the National Academy of Sciences, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Mapping of RNA by a modification of the Berk-Sharp procedure: the 5′ termini of 15 S β-globin mRNA precursor and mature 10 S β-globin mRNA have identical map coordinatesNucleic Acids Research, 1979