Changes in the Rate of Oxygen Consumption by Mouse Spleen Following Whole-Body X-Irradiation

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Abstract
Mice given single exposures of 500 R of X-irradiation were sacrificed at intervals up to 9 days after irradiation, and the C2 consumption of the chopped spleens was measured polarographically in Eagle''s basal medium. Og consumption dropped by about 30% over the 6 hr. following irradiation and rose again after 24 hr. to about 10% below the control value. A second minimum was found at 3 to 4 days. The wet-to-dry weight ratio of the spleen tissue showed no significant change. The drop in O2 consumption over the first 6 hr. is probably due to cellular damage, and the subsequent rise to removal of the damaged cells.