Use of Fluorophore-Conjugated Lectins To Study Cell-Cell Interactions in Model Marine Biofilms
- 1 January 2005
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 71 (1) , 428-35
- https://doi.org/10.1128/aem.71.1.428-435.2005
Abstract
Biofilms dominated by pennate diatoms are important in fields as diverse as ship biofouling and marine littoral sediment stabilization. The architecture of a biofilm depends on the fact that much of its mass consists of extracellular polymers. Although most illuminated biofilms in nature are dominated by phototrophs, they also contain heterotrophic bacteria. Given the close spatial association of the two types of organisms, cell-cell interaction is likely. Fluorophore-conjugated lectins were used to demonstrate the sites of the various extracellular polymers in three species of diatoms. Based on their lectin staining properties, the polymers in different species appeared to be similar, but their involvement in the process of attachment to a surface differed. In a coculture Pseudoalteromonas sp. strain 4 or its sterilized spent medium reduced the ability of Amphora coffeaeformis and Navicula sp. strains 1 and D to adhere, inhibited motility, and caused agglutination and eventually diatom cell lysis. Diatoms could be protected from the negative effects of the bacterial spent medium if D-galactose or mannan was included in the incubation medium. The active principle of the spent medium is probably a lectin/agglutinin that is able to bind to the extracellular polymers of the diatoms that are involved in adhesion and motility. Awareness of interactions of this type is important in the study of natural biofilms.Keywords
This publication has 44 references indexed in Scilit:
- Diatoms in BiofilmsPublished by Wiley ,2003
- Biochemical partitioning of photosynthetically fixed carbon by benthic diatoms during short-term incubations at different irradiancesMarine Ecology Progress Series, 2002
- Cell?cell and cell?surface interactions in an illuminated biofilm: Implications for marine sediment stabilizationPresented during the ACS Division of Geochemistry symposium ?Biogeochemical Consequences of Dynamic Interactions Between Benthic Fauna, Microbes and Aquatic Sediments?, San Diego, April 2001.Geochemical Transactions, 2001
- Analysis of algicidal proteins of a diatom-lytic marine bacterium Pseudoalteromonas sp. strain A25 by two-dimensional electrophoresisPhycologia, 2001
- Responses of microphytobenthos to light: primary production and carbohydrate allocation over an emersion periodMarine Ecology Progress Series, 2001
- Proteolytic enzymes in the marine bacterium Pseudoalteromonas atlantica: post-secretional activation and effects of environmental conditionsAquatic Microbial Ecology, 2000
- Isolation and characterization of extracellular polysaccharides from the epipelic diatomsCylindrotheca closteriumandNavicula salinarumEuropean Journal of Phycology, 1999
- Fouling Algae from 'In-service' ShipsBotanica Marina, 1986
- Interactions of Pseudomonas Aeruginosa Hemagglutinins With Euglena Gracilis, Chlamydomonas Reinhardi, and Tetrahymena PyriformisThe Journal of Protozoology, 1980
- ISOLATION AND PHYSIOLOGICAL STUDIES ON THREE ISOLATES OF AMPHORA (BACILLARIOPHYCEAE)1Journal of Phycology, 1976