RELATIONSHIP BETWEEN BETA-2 MICROGLOBULIN AND CELL-SURFACE ALLOANTIGENS OF MOUSE

  • 1 January 1976
    • journal article
    • research article
    • Vol. 117  (5) , 1532-1537
Abstract
Molecular relationships between .beta.2m [.beta.2-microglobulin] and other cell surface antigens (H-2, Tla, Ia [immune response associated antigen] and Thy-1) were studied with the double immunofluorescence method. Cells were incubated with an antiserum against 1 antigen, capped and then tested with an antiserum against a 2nd antigen. Capping of .beta.2m on thymocytes led to simultaneous capping of H-2 and Tla but not Thy-1 antigens; capping of H-2 and Tla (but not Thy-1) antigens resulted in capping of all .beta.2m detectable by the immunofluorescence method. Capping of .beta.2m on B [bone marrow-derived] or T [thymus-derived] lymphocytes resulted in capping of H-2 and vice versa. Ia antigens on B lymphocytes were not capped after the redistribution of .beta.2m. In the cell membrane of thymocytes, virtually all the .beta.2m molecules are associated with H-2 and Tla, but not with Thy-1. On the cell surface of T or B lymphocytes, virtually all .beta.2m is associated with H-2 but not with Ia. There was no evidence of any significant free .beta.2m on thymocytes or splenocytes.

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