The effect of peritoneal fluid on glucose‐U‐C14 oxidation by rat peritoneal leukocytes

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Abstract
The rate of C14O2 evolution from uniformly labeled glucose by washed white cells from peritoneal exudates increased several times after prolonged soaking of the cells in protein‐free medium and was repressed again upon incubation of the cells in peritoneal fluid of higher concentrations, whereas incubation in lower concentrations had a stimulatory effect, as compared to this rate by the cells incubated in protein‐free medium alone. Similarity of this effect of peritoneal fluid to the protective action of blood serum on the cells in tissue culture and the possible mechanism of action of both were discussed.It is suggested that the metabolism of the cells in multicellular organism is repressed by humoral macromolecular substances, and that the increase of glucose‐U‐C14 oxidation by the cells after prolonged soaking in protein‐free medium is related to the first step in the processes by which the cells move from a dormant state in vivo into that of active growth and cell divisions in vitro.