Isolation of indole-3-acetic acid from corn kernels & etiolated corn seedlings

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Abstract
Methods found useful in the isolation of IAA from kilogram quantities of plant tissue are described. A buffered ether-water partition column technique was developed. Trace amounts of IAA-2-C14 were used during the isolation and in evaluating the recovery. IAA was detected in 80% ethanol extracts of corn kernels and shoots, as well as vegetative sugar beet roots and leaves. The difficulties of detection of IAA in corn shoots are discussed in detail and it is suggested IAA may occur as a labile complex in ethanol extracts of this tissue. An 80% ethanol-soluble, aqueous-insoluble fraction from corn kernels was found to release IAA upon alkaline hydrolysis. It appears this fraction may be protein in nature.